Columns for Macromolecular Liquid Chromatography: Gel Filtration, Gel Permeation, Size Exclusion Chromatography Our stationary phases designed for Interaction Free GPC/SEC come in stainless steel columns of standard dimensions that fit any HPLC or LC (GPC, SEC, GFC) instrument.

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Column Selection Guide Facts and Tips

Columns for polymers soluble in water + salts/buffers, MeOH, acetonitrile Suprema         pH 2 - 12 Proteema        pH < 7  for proteins Novema          TFA, pH 1.5 -13 MCX                pH 1 - 14

Columns for polymers soluble in Organic Solvents SDV        THF, Toluene, Chloroform and Methylene chloride GRAM      for DMF, DMAC, NMP, DMSO    Purchase POLEFIN   for TCB, ODCB, Decalin PFG      Hexafluoroisopropanol (HFIP), Trifluoro ethanol, (TFE)

Columns for Light Scattering Detection SDVLux SupremaLux POLEFINLux  Lux columns reach the steady state mode > 25 times faster than conventional columns, and their overall noise reduction is more than 25 times better. Traditional GPC/SEC columns can produce excessive baseline noise from particulates in the eluent stream, which can significantly deteriorate the quality of the light scattering detectors data (especially LALLS/LALS)  Normally a new column set needs a 'conditioning' process, which involves flushing the columns to waste without any detector connected.

Mixed Bed Column vs. Column Combination Comparison linear column XL with a column combination for high molar masses One Linear Column: fast analysis time, broad molar mass range Column combination: high resolution

HighSpeed vs. Analytical Columns A HighSpeed column (red line) reduces the run time for a sample (broad Poly(styrene)) by a factor of 6 - 8 compared to a conventional column (black line). HS will substitute for analytical columns without modifications to your LC system, as long as pump and detectors accommodate 6.25 ml/min. At this flow rate, you will speed up the measurement using the same amount of solvent you currently use. Calibration with Poly(styrene) ReadyCal green, red, and white: each contains 4 different molecular weight standards PSS offers a wide variety of column dimensions and will manufacture custom sizes upon request.   Type                      I.D x Length (mm)   Guard columns        8 x 50; 4,6 x 30; 20 x 50         Analytical                8 x 300 and 8 x 600   Microbore                4,6 x 250   Preparative              20 x 600   HighSpeed               20 x 50 Analytical, HighSpeed and guard Columns may be purchased online.   Email us for a custom quote for analytical columns (8x600), Microbore and Preparative columns